Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here. we describe a protocol to monitor actin filament retrograde flow and actin polymerization within spines using a standard epi-fluorescence ... https://storeanimalwiz.shop/product-category/outdoor/