Abstract Focal adhesions (FAs) are flat elongated structures that mediate cell migration and link the cytoskeleton to the extracellular matrix. Along the vertical axis FAs were shown to be composed of three layers. We used structured illumination microscopy to examine the longitudinal distribution of four hallmark FA proteins. which we also used as markers for these layers. https://unitedssports.shop/product-category/hockey-protective-elbow-pads-senior/